Table 3 Primers used for the 5′ and 3′ junctions.

From: Identification of the Sex of Pre-implantation Mouse Embryos Using a Marked Y Chromosome and CRISPR/Cas9

Locus

Sequence (5′-3′)

products

5′F

ACCGTAAATACTCCACCC

1172 bp

5′R

GTCTGAAGACAGCTACAG

3′F

CTGCTGCCCGACAACCACT

1016 bp

3′R

ACCAGAAGAGGGCATCAGAT