Figure 4 | Scientific Reports

Figure 4

From: Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum

Figure 4

Expression of tdTom in schizonts of gexp02-Tom lines. (a) Representative flow cytometry analysis of the NF54-gexp02-Tom line at ~48–53 hpi (induced by choline depletion at the previous cycle and treated with ML10), showing presence of tdTom-positive schizonts (upper right gate), in addition to stage I gametocytes (upper left gate), and tdTom negative schizonts (lower right gate). Insets are live cell fluorescence microscopy images of the different subpopulations. (b) Representative IFA images of schizonts that are either tdTom positive or negative. All schizonts were Pfs16-negative. Scale bar: 5 µm. (c) Flow cytometry-based quantification of tdTom positive schizonts at the generation of induction and the following generation. Induced (choline-depleted) and uninduced (choline-supplemented) cultures, with or without addition of ML10, were tested. Data are presented as the average and s.e.m. of three independent biological replicates.

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