Figure 5

Transcriptional dynamics of the gexp02 gene. (a) Transcriptional analysis of gexp02 in the E5-HA-DD line, in which PfAP2-G is fused to the FKBP destabilization domain, cultured either without Shield 1 (Shld) (no gametocytes are formed) or with Shld added at 0–5 hpi to stabilize PfAP2-G. The inset graph in the –Shld panel shows the same data with a different y-axis range, because under these conditions gexp02 transcripts levels were very low. Transcript levels are normalized against ubiquitin-conjugating enzyme (uce) (PF3D7_0812600). (b) Schematic overview of the design of experiments aimed at comparing the transcriptional dynamics of pfap2-g and gexp02 in induced (choline-depleted) and uninduced (choline-supplemented) NF54-gexp02-Tom cultures. Analysis was performed at the schizont stage of the generation of induction (40–45 hpi, some new rings already present) and at the ring stage of the next generation (~0–10 hpi). Additionally, gexp02 transcript levels were determined in mature stage V gametocytes (Day 14). (c) Transcript levels of gexp02 and pfap2-g relative to the serine-tRNA ligase (serrs) gene (PF3D7_0717700). Data are presented as the average and s.e.m. of two independent biological replicates.