Figure 4

Multigenerational exposure to Cd in conjuncture with diet. Adult male and female C57BL/6J mice on defined, low-fat diets were exposed to drinking water containing 0, 0.5 or 5 ppm Cd for >2 weeks before being established into eight breeding triplets (F₀). F₀ mice (n = 8 ♂, n = 16♀ for each concentration) were continuously exposed to Cd during pregnancy. The offspring (F₁), four male and four female mice from each triplet were exposed to the same toxicants as their parents after weaning. At weaning, male (blue) and female (pink) offspring were fed either a low- (n = 8; light gray diamonds) or high-fat diet (n = 8; dark gray diamonds), respectively for 10 or 24 weeks. Hatched lines; F₀ mice.