Figure 3 | Scientific Reports

Figure 3

From: Basal Sodium-Dependent Vitamin C Transporter 2 polarization in choroid plexus explant cells in normal or scorbutic conditions

Figure 3

Choroid plexus explants maintain SVCT2 polarization. (A–C) Explants of choroid plexus analyzed by immunofluorescence and confocal microscopy after anti-SVCT2 or anti-MCT1 incubation. TOPRO-3 was used for nuclear staining. SVCT2 showed basolateral polarization (Bas), and MCT1 was detected in apical membranes (Ap). (D) Immunofluorescence and confocal microscopy 3D-reconstruction of choroid plexus after anti-ZO1 or SVCT2 incubation (3D). Confocal orthogonal microscopy reconstruction for ZO-1 and SVCT2 identification in three different regions inside the epithelial cell bilayer (1,2,3). (E,G,I) Immunofluorescence and confocal microscopy 3D-reconstruction of choroid plexus from the lateral and fourth ventricles after incubation with different combinations of antibodies: anti-SVCT2 and anti-GLUT1 (E,F), anti-GLUT1 and anti-MCT1 (G) or anti-GLUT1, anti-MCT1 and anti-SVCT2 (H). High-power imaging of confocal spectral microscopy analysis is showed in I and J. GLUT1 and SVCT2 showed the higher colocalization in the basal membrane (J, arrows). All images are representative of different biologically independent samples. A–D, n = 9. E–J, n = 9. Asterisk. Connective tissue. Scale bars: A 100 μm; B, D, G, H 30 μm; C, E, F 20 μm; E–H 50 μm; I–J 8 μm.

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