Figure 4 | Scientific Reports

Figure 4

From: Basal Sodium-Dependent Vitamin C Transporter 2 polarization in choroid plexus explant cells in normal or scorbutic conditions

Figure 4

SVCT2 is expressed early during brain development and choroid plexus cell polarization appears after 10 postnatal days. (A) In situ hybridization analysis of embryonic 17-day-old brains. The choroid plexus of the fourth ventricle had higher SVCT2 mRNA expression. (B) In utero electroporation analysis using the pEYFP-N1/hSVCT2wt vector in embryonic day 15. Immunofluorescence analysis after anti-GLUT1 incubation; Topro-3 was used for nuclear staining. Merged image indicates that over-expressed SVCT2 is polarized in the basolateral membrane (arrow). (C–E) Explant of choroid plexus at postnatal days 5, 10, 15 and 20, analyzed by immunofluorescence and confocal microscopy after anti-SVCT2 and anti-ZO1 incubation (C), anti-SVCT2 and anti-MCT1 incubation (E) and anti-SVCT2 and anti-GLUT1 incubation (D). TOPRO-3 was used for nuclear staining. All images are representative of different biologically independent samples. A, n = 3. B, n = 6. C–E, n = 4. 4v. Fourth ventricle. CP. Choroid plexus. E. Endothelial cells. LV. Lateral ventricles. Scale bars: A 100 and 50 μm (lower magnification) and 15 μm (higher magnification); B 20 μm; C–E 50 μm (lower magnification) and 15 μm (higher magnification).

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