Figure 6 | Scientific Reports

Figure 6

From: Basal Sodium-Dependent Vitamin C Transporter 2 polarization in choroid plexus explant cells in normal or scorbutic conditions

Figure 6

Vitamin C deficiency and normal energetic culture demands increase SVCT2 basolateral polarization in choroid plexus cells from Guinea pig. (A) Scanning electron microscopy of choroid plexus explants after induction of vitamin C deficiency. (B) Confocal microscopy analysis of choroid plexus explants maintained in normal or vitamin C deficiency conditions after incubation with anti-ZO. TOPRO-3 was used for nuclear staining. (C) Confocal microscopy analysis of choroid plexus cells after anti-SVCT2 and anti-GLUT1 incubation in normal or vitamin C deficiency conditions. (D) Glucose and lactate induce SVCT2 re-polarization in the basolateral membrane of choroid plexus cells cultured without glucose (7 h). All images are representative of different biologically independent samples. A, n = 2. B, n = 2. C–D, n = 6. Scale bars: B 30 μm (lower magnification) and 5 μm (higher magnification); C 50 μm (lower magnification) and 20 μm (higher magnification); D 20 μm.

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