Figure 5

Following injury, OPG/sRANKL ratios and IL-6 were significantly elevated in FVIII^−/− and FIX^−/− mice compared to injured WT littermate controls. (A) sRANKL was significantly lower in injured FVIII^−/− and FIX^−/− mice in comparison to injured WT controls and naïve knockout mice, respectively. (B) OPG was significantly elevated in injured FVIII^−/− and FIX^−/− mice in comparison to injured WT mice, and in injured vs naïve FIX^−/− mice. (C) OPG/sRANKL ratios were significantly higher in injured FVIII^−/− and FIX^−/− mice in comparison to both naïve knockout and WT littermate controls. (D) IL-6 levels were significantly higher than injured WT controls. Average ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. FVIII^−/− groups (Non-injured: Naive n = 14/FVIII^−/− n = 27; Injured: Naive n = 14/FVIII^−/− n = 33); FIX^−/− groups (Non-injured: Naive n = 13/FIX^−/− n = 13; Injured: Naive n = 18/FIX^−/− n = 27). Number of outliers removed: FVIII^−/− groups (Non-Injured: Naive n = 1; Injured: Naïve n = 3; FVIII^−/− n = 2); FIX^−/− groups (Injured: Naive n = 5/FIX^−/− n = 4).