Table 1 Comparison of congenital differences in FVIII, FIX, and VWF lines of mice.

From: Hemophilia A and B mice, but not VWF−/−mice, display bone defects in congenital development and remodeling after injury

 

FVIII−/−

FIX−/−

VWF−/−

WT

KO

Significance

WT

KO

Significance

WT

KO

Significance

Trabecular μCT Parameters

vBMD (mgHA/cm3)

174.9096 +/− 12.149

126.4247 +/− 10.333

0.0057

181.8782 +/− 10.565

153.7318 + /− 8.157

0.0447

172.7436 +/− 11.854

179.0456 +/− 10.382

0.7028

Trabecular Bone Volume Fraction BV/TV (%)

0.1445 +/− 0.011

0.1038 +/− 0.0009

0.0080

0.1468 +/− 0.010

0.1227 +/− 0.008

0.0654

0.1378 +/− 0.011

0.1404 +/− 0.011

0.7445

Connectivity Density (mm3)

81.9748 +/− 9.970

54.565 +/− 7.649

0.0418

114.9248 +/− 10.401

84.3252 +/− 7.693

0.0258

111.0571 +/− 8.879

120.4066 +/−  10.003

0.5323

Trabecular Thickness (mm)

0.0530 +/− 0.0002

0.0503 +/− 0.002

0.2728

0.0454 +/− 0.001

0.0457 +/− 0.002

0.5795

0.0436 +/− 0.0001

0.0447 +/− 0.001

0.5537

Trabecular Number (mm-1)

4.2003 +/− 0.146

3.5781 +/− 0.165

0.0084

4.916 +/− 0.127

4.2954 +/− 0.177

0.0082

4.7223 +/− 0.121

4.9193 +/− 0.095

0.2166

Trabecular Spacing (mm)

0.2352 +/− 0.010

0.2843 +/− 0.016

0.0110

0.1963 +/− 0.006

0.2328 +/− 0.011

0.0081

0.2050 +/− 0.006

0.1948 +/− 0.005

0.2039

Cortical mCT Parameters

Cortical Thickness

0.2175 +/− 0.003

0.2197 +/− 0.004

0.6642

0.2019 +/− 0.003

0.1960 +/− 0.003

0.1535

0.1940 +/− 0.005

0.1925 +/− 0.003

0.7643

Cortical Porosity

3.6481 +/− 0.107

3.6946 +/− 0.044

0.2717

4.1843 +/− 0.073

4.4157 +/− 0.218

0.3236

2.914 +/− 0.567

2.400 +/− 0.411

0.4628

Marrow Cavity Area

0.9762 +/− 0.0309

0.9014 +/− 0.32

0.1036

1.0182 +/− 0.048

1.0330 +/− 0.054

0.8384

1.2221 +/− 0.031

1.1087 +/− 0.031

0.0217

Polar Moment of Inertia

0.4581 +/− 0.020

0.4152 +/− 0.018

0.1215

0.4328 +/− 0.027

0.4137 +/− 0.026

0.6149

0.5228 +/− 0.20

0.4614 +/− 0.017

0.0293

Surface of Articulating Bone Ratio of Smoothness

0.8989 +/− 0.003

0.8942 +/− 0.007

0.5404

0.9049 +/− 0.004

0.9099 +/− 0.003

0.3529

0.9179 +/− 0.002

0.9127 +/− 0.002

0.1635

DXA Parameters

BMD 16 weeks

0.0552 +/− 0.001

0.0533 +/− 0.001

0.0325

0.0529 +/− 0.001

0.0505 +/− 0.001

0.0057

0.0536 +/− 0.001

0.0541 +/− 0.001

0.8318

BMD 22 weeks

0.0562 +/− 0.001

0.0546 +/− 0.001

0.1010

0.0541 +/− 0.001

0.0530 +/− 0.001

0.3946

0.0538 +/− 0.001

0.0551 +/− 0.001

0.2229

BMC 16 weeks

0.4692 +/− 0.009

0.4412 +/− 0.007

0.0500

0.4106 +/− 0.100

0.3778 +/− 0.007

0.0113

0.4477 +/− 0.008

0.4558 +/− 0.007

0.8784

BMC22 weeks

0.4889 +/− 0.009

0.4674 +/− 0.007

0.1566

0.4306 +/− 0.009

0.3962 +/− 0.006

0.0068

0.4625 +/− 0.008

0.4674 +/− 0.010

0.9685

  1. µCT measurements were compared using t-test comparison between WT and KO of each line. FVIII WT n = 16, KO n = 14; FIX WT n = 14, KO n = 14; VWF WT n = 10, KO n = 17. DXA measurements BMD and BMC were compared using 2-way ANOVA with multiple comparisons. FVIII WT n = 26, KO n = 34; FIX.
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