Figure 1 | Scientific Reports

Figure 1

From: A novel form of Deleted in breast cancer 1 (DBC1) lacking the N-terminal domain does not bind SIRT1 and is dynamically regulated in vivo

Figure 1

Quiescent cells dynamically express a low molecular weight form of DBC1 that lacks the N-terminal region. (A) WT and DBC1 KO MEFS were maintained either in 10% FBS (+) or in Serum free media (−) for 48 hours. Western blot for DBC1 was performed using an antibody for the C-terminal domain (sequence surrounding aa 875) or an antibody for the N-terminal domain (aa 1–50). Black arrows point to DBC1. Red arrows show the lower molecular weight band. (B) WT MEFS were incubated in serum-free media for different times. (C) SIRT1 activity was measured in WT and DBC1 KO MEFs at time 0 or after 24 hours of serum deprivation. *means p < 0.05 compared to Control (t0) for each genotype, unpaired t-test. (D) WT and DBC1 KO MEFS were incubated either in the presence of 10% FBS (C) or in serum-free media (−) for 48 hours. At time 0, media was replaced for 10% FBS (+) in quiescent cells and cells were collected at different time points. (E) SIRT1 activity was measured in WT and DBC1 KO MEFs at time 0 (48 hours of serum deprivation) or after 12 hours of serum (10% FBS) replenishment. *means p < 0.05 compared to Control (t0) for each genotype, unpaired t-test.

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