Figure 5

DBC1 KO mice have impaired liver regeneration after partial hepatectomy. (A,B) The cyto-architecture of control (sham) and regenerating livers (PH) differ between WT and DBC1 KO mice. (A) Lobule organization seems to be similar in intact (Sham) livers of WT and KO mice. However, after 72 hours of partial hepatectomy (PH) the lobule organization around the central vein (thick arrows) is almost complete in WT livers. Note that sinusoids (thin arrows) that are being formed in WT livers, are difficult to trace in lobules of DBC1 KO animals. Bar is 200 micrometers. (B) Higher magnification of livers after 72 hours of partial hepatectomy, showing a lobular central vein (thick arrow) and hepatocytes surrounding it to better appreciate the presence of newly formed sinusoids (thin arrows) in WT livers and their absence in KO ones. Bar is 50 micrometers. (C) Aspartate aminotransferase (AST, upper panel) and Alanine aminotransferase (ALT, lower panel) activity measured in plasma of WT and DBC1 KO mice during liver regeneration. *p < 0.05, two-way ANOVA (D) Time course of liver mass recovery after partial hepatectomy in WT and DBC1 KO mice. Liver mass was normalized to total body weight. *p < 0.05 Unpaired t-test at each time point. Total liver mass was not different between WT and DBC1 KO in sham control mice.