Figure 4
From: Oral selective serotonin reuptake inhibitors activate vagus nerve dependent gut-brain signalling
Sertraline, but not vagotomy, induce a decreased microbial richness. (A,B) Effect of sertraline treatment on the change in alpha-diversity from baseline to post-treatment: (Simpson index, Mann-Whitney U = 31, p = 0.017; Shannon index, Mann-Whitney U = 31, p = 0.017), 4675 reads/sample. (C) Bray-Curtis distances from rarefied 16S rRNA data (4675 reads/sample) comparing distances between baseline and post-treatment time-points for control and sertraline-treated mice (Mann-Whitney U = 55, p = 0.343). (D,E) Effect of vagotomy versus sham surgery on alpha-diversity metrics: (Simpson index, Mann-Whitney U = 17, p = 0.074; Shannon index, Mann-Whitney U = 19, p = 0.113), 36040 reads/sample. (F) Bray-Curtis distances from rarefied 16S rRNA data (36040 reads/sample) comparing within-group distances of sham surgery and vagotomised groups, and showing distances between sham and vagotomised groups (Mann-Whitney U = 457, p = 0.529). (G) Principal coordinates analysis (PCoA) plots of Jackknifed Bray-Curtis dissimilarity distances from rarefied 16S rRNA data (n = 999 rarefactions, 36040 reads/sample) with PERMANOVA analysis revealing a lack of clustering of samples by group as a result of vagotomy (F = 1.989, p = 0.082), (red: sham; blue: vagotomy). *P < 0.05.
