Figure 2 | Scientific Reports

Figure 2

From: HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation

Figure 2

In vivo RPA2 ubiquitination mediated by C-terminus of HERC2. (a) HeLa-shHERC2 cells were induced or not with Dox, treated with MG132, and subjected to immunoprecipitation in denature condition followed by immunoblotting with the indicated antibodies. Inputs were also loaded. (b) HeLa-shHERC2 cells were co-transfected with St2-RPA2 and HA-ubiquitin (HA-Ub), induced or not with Dox, treated with MG132, and subjected to Strep-Tactin pulldown followed by immunoblotting with anti-HA antibody to detect ubiquitinated RPA2 products. (c) HeLa-shHERC2 cells were co-transfected with the indicated plasmids, induced with Dox, treated or not with MG132, and ubiquitinated RPA2 products were detected as in (b). The asterisk indicates non-specific band. Myc-F5: Myc-HERC2-F5.

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