Figure 6
Epistatic relationship of RPA1 and HERC2 or its E3 activity on G4 formation. (a,b) HeLa-shHERC2 cells were transfected with the indicated siRNAs, induced or not with Dox and subjected to immunoblotting with the indicated antibodies (a) or immunostained for G4 (b). (c) Quantification of the mechanically counted G4 foci per cell is shown. Error bars, SEM of duplicate experiments, each based on more than 100 cells. (d,e) Wild-type or HERC2ΔE3/ΔE3 HCT116 cells were transfected with the indicated siRNAs and subjected to immunoblotting with the indicated antibodies (d) or immunostained for G4 (e). (f) Quantification of the G4 foci was performed as in (c).
