Figure 4
From: Three-Dimensional Microscopy by Milling with Ultraviolet Excitation
Central profiling of microspheres using a wide-field fluorescence microscope (blue) and MUVE (green). The axial measurements of micro-beads (≈4 μm) were collected at a 1.0 μm sectioning size for both optical and physical sectioning. Intensity plots were measured across the central line along the cutting direction indicated by a white arrow. 3D volume rendering of large beads (≈500 μm) showing imaging artifacts such as shadows (indicated by black arrows) involved in optical sectioning microscopy.
