Figure 8
From: Three-Dimensional Microscopy by Milling with Ultraviolet Excitation
Coronal MUVE imaging of mouse thalamus stained with India-ink and thionine. (a) Tissues are dark red while cell nuclei are dark brown (arrow) and vessels are black (dashed arrow) under UV illumination (left), providing enough contrast to segment both the cellular and vascular structures (right). (b) Volume rendering of the entire data set shows the density and organization of microvasculature with surrounding cellular details. (c) Volume rendering of a small region (100 × 100 × 100 μm) shows series of connected microvessels (red) along with their associated cells (green), and separate channels show detailed cellular and vascular structures.
