Figure 5
TFEB represses the expression of DCAF11. (a) The mRNA levels of Keap1, β-TrCP, DCAF11 and Hrd1 were analyzed by qRT-PCR as described in Methods. Bar graph represents the relative mRNA levels of Keap1 and Nrf2-specific E3 ubiquitin ligase genes in H4 cells stably expressing TFEB (TFEB) compared to H4 cells (Control). (b) The protein levels of Keap1 and Nrf2-specific E3 ubiquitin ligases in H4 and TFEB cells were analyzed by immunoblotting using each corresponding antibody. Full blots are provided in Supplementary Fig. S10. Data shown are mean ± S.E. of three independent experiments and were analyzed using Student’s t test. (**p < 0.01; ***p < 0.001).
