Figure 6
TFEB-mediated DCAF11 repression is dependent on the CLEAR element on its 5′-UTR. (a) shows a schematic drawing of luciferase-reporter plasmids with 5′-UTR of DCAF11 gene having wild-type or mutant CLEAR element sequence. One CLEAR element is present at +242 ~ +247 from the transcription start site (TSS, +1). WT, wild-type; MT, mutant. (b) H4 cells (Control) and H4 cells stably expressing TFEB (TFEB) were transiently transfected with the indicated plasmid, and the luciferase activity was assayed at 18 h after transfection. Data shown are mean ± S.E. of four independent experiments and were analyzed using Student’s t test. (***p < 0.001).
