Figure 1
(a) Study design. Schematic of study outline with pairs of animals administered virus via the subcutaneous (S/c) route; gender indicated as M (male) or F (female). Rhesus macaques (RM), cynomolgus macaques (CM), red-bellied tamarins (tamarin) or marmosets were challenged and sacrificed after 3 (red), 42 (blue) or 100 days (green). (b) Acute viral kinetics in blood post ZIKV PRVABC59 sub-cutaneous inoculation. Viraemia profiles for each time-course experiment with viral RNA levels for each species depicted relating to the day of termination: day 3 (red), day 42 (blue) and day 100 (green), up to and including the day 42 bleed. Reproducibility of sampling for each species shown as a composite representing the different time-courses. Viral RNA levels are expressed as ZIKV RNA copies/ml determined from serum for marmosets and tamarins (New World) and plasma in rhesus and cynomolgus macaques (Old World). All samples assayed beyond 42 days were below the level of detection (50 ZIKV RNA copies/ml).
