Figure 2

Tissue distribution of ZIKV RNA in New and Old World monkeys determined by qRT-PCR. Tissue viral RNA levels in materials collected post-mortem following total tissue RNA extraction, expressed as ZIKV RNA copies per µg total RNA normalised to a GAPDH house-keeping gene. Days 3 and 42 are compared for each species. Tissues are broadly grouped according to anatomical region - skin: (inoculation site, thigh, ankle, wrist, shoulders, back, chest, abdomen); nerve tissue: (sural, sciatic, ulnar, median, radial, dorsal root ganglion (DRG)); Lymph node (LN): peripheral lymph node (PLN), - inguinal lymph node (ILN), basal lymph node (BLN), mesenteric lymph node (MLN), spleen, salivary gland lymph node; secretory sites: salivary gland, tear ducts, kidney, bladder, ureter, urethra; reproductive sites: genital lymph node (LN), penis, testes, vas deferens, prostate, cervix, uterus, vagina, ovaries; brain tissue: cerebrum/frontal lobe, cerebellum, cortex, mid-brain.