Figure 3
(a) Relationship between tissue vRNA and blood vRNA levels in individual Old and New World monkeys in lymphoid tissue 3 days p.i. Symbols representing each tissue sampled are shown in the key. PLN (ILN/BLN); peripheral lymph node (inguinal/basal); MLN, mesenteric lymph node; sal glnd LN (salivary gland lymph node). Colour code: marmosets (dark red), tamarins (red), Indian rhesus macaque (blue) and Mauritian cynomolgus macaque (light blue). (b) Comparative RNAscope analysis of ZIKV RNA tissue distribution. The frequency and intensity of staining frequencies are indicated by the heat-map coding as shown. Localised areas of staining summarised in the table are abbreviated as follows: Adp, adipose layer; RD, reticular dermis; HBb, hair bulb base; ERS, external root sheath; BsL, basal layer; ML, molecular layer; GL, granular layer; GM, grey matter; PC, paracortex; mgmz, marginal/mantel zone; 1°, primary follicle; 2°, secondary follicle; GC, germinal centre; Musc C, muscularis circular muscle layer; LP, laminar propria; PCL, prickle cell layer; Hep, hepatocytes; KC, Kupffer cells; DM, dendritic morphology; TCR, T cell regions, FCS, fibrocollagenous stroma; LEC, luminal epithelial cells; I/OTC, inner and outer tubule cells; OTC, outer tubule cells; LC, Leydig cells; S’gonia, spermatagonia; NB, neuronal body. WM, white matter. GLN, genital lymph node, Small Int, small intestine. (c) RNAscope localisation of ZIKV RNA in lymphoid tissue (mesenteric lymph node) Representative images of RNAscope detection of ZIKV RNA within FFPE MLN collected post-mortem from either Indian rhesus macaques and red-bellied tamarins 3, 42 and 100 dpi. Main image x20, inset x40 magnification. Characteristic brown stained ZIKV RNA positive cells (circled x20 images) identified within follicular germinal centres, marginal zones and paracortical regions of MLN were present 3 dpi remaining detectable 42 and 100 dpi.
