Figure 6
Persistence of ZIKV RNA in male tamarin reproductive tissue. Representative images of RNAscope detection of ZIKV RNA within FFPE tissues from male tamarin reproductive organs collected 3 and 42 days post infection (dpi). Testes sections a–c. (a) Main image x20, inset x40 magnification of brown stained ZIKV RNA positive cells identified as discrete foci in seminiferous tubules within sections of testes at 3 dpi (x20 magnification, tamarin P6), with more intense staining out to 42 dpi (panel b) with multiple foci covering a more extensive area (tamarin P1). (c) Comparative analyses for 100 dpi in the rhesus macaque as a single, large focus of ZIKV RNA expressing cells identified as persistent infection. In both species virus occurs in the outer layers containing primary spermatocytes. Evidence of staining in other parts of male reproductive tissue compared for tamarin P5 (3 dpi) and tamarin P1 (42 dpi) as shown in panels d,e) prostate, 3 and 42  dpi, and (f,g) genital lymph node (LN), with foci persisting at 3 dpi (tamarin P5) and more widespread at 42 dpi (tamarin P1).
