Figure 3
From: An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances
Toxicity detection of substance mix and drug. (a) Workflow to measure effects of a substance mix and drugs. Uremic toxin mix was applied in Cmax (highest concentration measured in uremic patients) and in Clow (1:16 dilution of Cmax). Following substances and concentrations were applied within the UTM: Uric acid (Cmax: 147 µg/ml; Clow: 9.19 µg/ml), xanthine (Cmax: 3.44 µg/ml; Clow: 0.22 µg/ml), uridine (Cmax: 32.6 µg/ml; Clow: 2,04 µg/ml) and uracil (Cmax: 0,45 µg/ml; Clow: 0.03 µg/ml). (b) Survival (left) and motility (right) were analyzed after 120 minutes of incubation on a minimum of 3 donors. (c) Motility analysis of five experimental rounds. Cells from three different donors. (d) Frequency of viable cells over time on cells from three donors. In (c) and (d) SEM are shown. (e) Furosemide was applied to the cell model to test for motility. Incubation of 120 minutes was performed.P-value indicates significance level.
