Figure 4
From: An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances
Toxicity detection of denominated uremic toxins (UT). (a) Workflow to measure effects of single substances (S1-Sx). (b) 47 UT tested for influence on motility (after 90 minutes of incubation) and survival (after 300 minutes of incubation). UT are assigned to the three bio-chemical classes (small water-soluble compounds (SWS), protein bound (PB), middle molecules (MM)). Data are shown as relative change to non-toxic control. M1 and M2 represent two separate experimental motility rounds, V represents mean viability of six experimental rounds. Bold lines mark UT with reduced motility <25%, dashed lines mark UT with increased motility >25%. Marked UT are displayed in (c). Left: all UT with toxic effect, right: all UT with non-toxic effect. At least two separate experimental rounds are shown. Incubation was performed for 90 minutes.
