Table 4 PCR primers for sex identification and an internal control, NADH dehydrogenase subunit 4 (ND4) gene.
Primer pair | Name | Sequence (5′ to 3′) | Size (bp) |
|---|---|---|---|
Pair I | sca64_3724604_F | TGCACCTGTAACTCACTAACCG | 113 |
sca64_3724604_R | CCTTTTCCTGGCCTCTTTACAT | ||
Pair II | sca64_3726411_F | GCAGACAAAAAGCCATTCG | 143 |
sca64_3726411_R_A* | CTGATGACCTCTGTAACACAATCAT | ||
sca64_3726411_R_T* | CTGATGTCCTCTGTAACACAATCAT | ||
Pair III | sca64_3724591_F | CAGAAATCACCAGTGCACC | 142 and 149 |
sca64_3724591_R | GGATATTATTAGGAAACCTTTTCCTG | ||
Pair IV | ND4_F | ACAGACCCGTTGTCAACTCC | 268 |
ND4_R | TCCCTGCATTTAAACGCTCT |
