Figure 1
Design of the microfluidic transduction device. (a) Photograph of the 2M-MTD (US quarter for size reference). (b) Cartoon of the 2M-MTD identifying major design features including the primary transduction chamber and microfluidic flow channels. (c) Cartoon for the MTD transduction protocol (i) Target cells and virus are introduced into the device using microfluidic flow down through the semi-permeable membrane, trapping the cells and virus in the transduction chamber. (ii) During transduction, transmembrane fluidic flow pins the cells and virus on the surface of the membrane, increasing the rate of interaction between the two particles. (iii) To recover cells, transmembrane fluidic flow is reversed up through the membrane while additional fluid is pushed across the membrane, driving collection of cells and virus through the recovery channel. (d) CFD prediction of wall-shear stresses are low and not expected to negatively impact cell health. (e) Top-down view of transduction chamber (only half shown) illustrating CFD predictions of target cell’s point of contract with the porous membrane. The distribution is largely uniform across the membrane surface. (f) Analytical model predictions of total relative virus found ≤10 microns of the porous membrane, plotted vs. transmembrane flow rate. (g) Analytical model predictions for relative virus concentration in a singular plane 5 microns above the membrane, plotted vs. transmembrane flow rate.
