Figure 3
The MTD can be scaled without loss of functionality. 10 million activated T cells were transduced in the 10M-MTD and compared to cells transduced in the 2M-MTD and to static controls. (a) Photograph of the 10M-MTD next to the 2M-MTD (US quarter for size reference). (b) Transduction efficiency for repeat experiments following 90 minute and overnight static transduction or 90 minutes MTD transduction at MOI of 2.5. (c) Average percent of T cells recovered from the 10M-MTD. (d) T cell viability relative to static controls immediately following removal from the 10M-MTD and wash in media. (e) T cell viability measured by 7AAD and flow cytometry for cells expanded 4 days after transduction. All panels represent data from n = 3 devices with T cells derived from N = 3 different donors. All error bars indicate standard deviation.
