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Figure 1

From: Low human and murine Mcl-1 expression leads to a pro-apoptotic plaque phenotype enriched in giant-cells

Figure 1

Regulation of Mcl-1 expression in atherosclerosis. (A–C) Mcl-1 gene expression measured by RT-qPCR. (A) Vascular Mcl-1 expression corrected for HPRT housekeeping gene in a model of collar induced carotid artery atherogenesis in LDLr−/− mice. (B) Mcl-1 expression corrected for 18 S housekeeping gene in different mouse cell types. SMCs: smooth muscle cells, MCECs: mouse cardiac endothelial cells. (C) Mcl-1 gene expression in human atherosclerotic plaques, represented by microarray normalized intensities. (D) Heatmap showing Pearson correlation coefficient (p-values) of Mcl-1 human plaque gene expression correlation with clinical plaque traits. N = 22/23 (stable/unstable). (E) Lethally irradiated LDLr−/− mice were reconstituted with WT or Mcl-1−/− bone marrow, and after 8 weeks of recovery, put on a Western Type Diet (WTD) containing 0.25% cholesterol for 5 weeks (n = 17) or 10 weeks (n = 19). All data is presented as mean ± SEM. *p < 0.05.

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