Figure 4

3D quantification of tumour volume and tumour vascularization. (A–C) Quantification of the tumour volume. The 3D projection of the scattered light (red) collected from the RT2 tumour (A) was used to define the tumour surface and base area (D–I) Multi-step process of quantification of the tumour vasculature. The 3D tumour projection was used to define the tumour vasculature (green) (D) in order to remove the iris vasculature (E). The fluorescent signal was thresholded to obtain a binary 3D image (F), used to create a digital skeleton representing the vasculature (G). The skeleton facilitated quantification of various properties such as Branch and End Points (H), segment diameter, length and number (I).