Figure 6

VEGF-B overexpression reduces tumour growth but increases tumour angiogenesis. (A) Image panels showing representative RT2 and RT2-VEGF-B tumours at indicated time-points. Tumour cells were visualized by scattered light (grey scale) and tumour vessels by FITC-500 kDa dextran administered to the circulation (FITC) (grey scale). Bottom row represents merged images. Scale bars represent 50 μm. (B) Graphs showing the growth of individual tumours during the study. (C) Fold changes in tumour volume during the study. The population of non-growing tumours (<2 fold growth) was 20% in the RT2 group and increased to 58% in the RT2-VEGF-B group (RT2 = 20, RT-VEGF-B = 25). (D) Quantification of indicated tumour vascular parameters stratified into non-growing tumours (<2 fold growth) and growing tumours (>2 fold) (RT2 = 20, RT-VEGF-B = 25). Statistics: (C) Student’s t-test mean ± SD, (D) Mann-Whitney-Wilcoxon Test, median ± SD; *P < 0.05, **P < 0.01.