Figure 1
IFNT stimulated STAT1 dependent type-1 interferon pathway in LGCs. (a–c) Cells were incubated for 24 h with either basal media (control) or varying concentrations of roIFNT (0.01–10 ng/mL). Cells were then harvested, and the mRNA expression of (A) MX2, (b) ISG15 and (C) OAS1Y were determined using qPCR. (d) Tyrosine phosphorylated STAT1 (p-STAT1) and (e) total STAT1 proteins were analyzed using specific antibodies in western blotting. Representative images of western blots are presented in the upper panels of D and E. Blots in (d) were cropped from different gels, blots in (e) were cropped from different parts of the same gels. Whole cell extracts from LGCs were incubated with IFNT (1 ng/mL) for different time points as indicated. Quantitative analysis of phospho-STAT1 levels were normalized to total STAT1 and total STAT1 was normalized to total MAPK (p44/42) protein. Asterisks indicate significant differences from either time 0 or basal media (control) or control (*P < 0.05, **P < 0.01, ***P < 0.001).
