Figure 4

Time-dependent effects of IFNT on pro- and antiapoptotic proteins. LGCs were incubated without (control) or with roIFNT (1 ng/mL) for 48 h, (a) MCL1, (b) BCL-xL, and (c) gH2AX protein levels were determined in cell extracts by western blotting and normalized relative to the abundance of total MAPK (p44/42). All blots expect MCL1 were cropped from different parts of the same gels. Results are presented as the means ± SEM from four independent experiments. (d) Representative images of the western blots for each antibody. Asterisks indicate significant differences from their respective controls (*P < 0.05, **P < 0.01, ***P < 0.001).