Figure 4

Relative gene expression of all eight expressed Bna.FLC copies in leaves before vernalisation 7 weeks after sowing (BBCH 14), and without and with vernalisation 15 weeks after sowing in the accessions Manitoba, Lisabeth (winter-type), Mansholt (winter-hardy, but not vernalisation-dependent), Girasol and Korall (both spring-type). The values were calculated from RT-qPCR using the ΔCt method and represent 3 biological replicates. Whiskers show SEM. “NE” stands for “not expressed”. Small letters represent group by LSD test. The scale is the same for all 3 plots.