Figure 7
Relationships between the broken reading frame found to be correlating with Bna.FLC.A10 expression. (A) Boxplots for relative gene expression of Bna.FLC.A10 and Bna.FLC.C02 in 48 spring and 53 winter accessions in leaves of 7 week old plants. (B) Relative copy number (RCN) of the region containing the sequence variant as a proxy for the presence of the sequence variant plotted against Bna.FLC.A10 relative gene expression. The dotted lines separate “low” and “high” RCN/ gene expression. (C) Sequence alignment of the unaffected (accession Alesi) and affected (accession Robust) contig assembly with the Darmor-bzh-5′UTR, the Darmor-bzh exon 1, the Tapidor full length sequence from39, the A. thaliana COOLAIR sequence and the sequence of the conserved 26 bp motif (indicated by blue frame). The site of the broken reading frame is also indicated. The blue arrow shows the ATG start site.
