Figure 6

PhPEBPs regulate seed germination in Arabidopsis. (a) Time course of germination rate for wild type Arabidopsis and 3 representative PhFT5 overexpression lines in the presence of 10 μM ABA. Bars represent SD from three independent biological repeats. (b) Overexpression of PhFT5 rescues low seed germination rate of mft-3 mutant in the presence of 10 μM ABA in Arabidopsis. Bars represent SD from three independent biological repeats. (c) PhFT5 expression dynamics in moso bamboo seed in the absence (blue) or presence (red) of 100 μM ABA. Bars represent SD from three independent biological repeats (n = 50). (d) Comparison of seed germination among wild type Arabidopsis and the overexpressors of PhFT5 and other PhPEBPs in the presence of 10 μM ABA. Bars represent SD from three independent biological repeats (n = 50). Seeds sowed on ½ MS (0.8% agar) medium with ABA kept in the dark at 4 °C for 3 days for stratification, and then transferred to 16 h light/8 h dark photoperiod at 21 °C for 5 days. *, ** and *** denote statistically significant differences determined by Student’s t test (P < 0.05), (P<0.01) and (P < 0.001), respectively.