Figure 4
(a,b) Two sets of replica images of M. pneumoniae attached on silica beads. The cells located at the furthest deep-etched area from the center of the beads (indicated by white arrowheads) were observed. They are shown as enlarged views (upper-left insets). Further enlarged stereo-views (center) exhibit numerous stump-like structures on the back. Their diameter matched very nicely with that estimated by cryo-electron-tomography30. Side-views of the same structure (black arrowheads) are observed along the edges of the cell-body, some of which might be, at least, very close or potentially in contact with the substrate. Scale-bars for field-views (left panels) and enlarged-views (right panel) indicate 500 nm and 100 nm, respectively. (c) Further enlargement of the grazing view along the left edge of the cell in (a). Side- or deeply-oblique views of stump-like structures are clearly visible along the line indicated by a white arrow. (d) Similar grazing view of the control-cell which is less inclined against the view-angle. Stump-like structures might be barely visible along the periphery but far less conspicuously than in (c). Scale-bar exhibits 10 nm for (c,d).
