Figure 4
From: A large intragenic deletion in the CLCN1 gene causes Hereditary Myotonia in pigs
(a) Schematic representation of the region between introns 14 and 16. Black arrows indicate primers used in the PCR-based genotyping assay. Red arrows represent the boundaries of the deleted region. Amplicon sizes are indicated for both wild-type and mutant alleles. (b) Cropped picture of the 1.5% agarose gel electrophoresis showing typical results of the genotyping assay for Hereditary Myotonia in pigs. Well 1: Molecular-weight size marker. Well 2: Homozygous dominant (pig C1 of the control group) result (868 bp amplicon). Well 3: Heterozygous (pig K) result (868 bp and 458 bp amplicons). Well 4: Homozygous recessive (pig E) result (458 bp amplicon). Well 5: Negative control. Blue arrows indicate the DNA ladder’s fragment sizes 900 bp and 500 bp.
