Figure 10

Secretory factors from L. reuteri CM treated T cells enhances osteoblast viability and osterix gene expression. Mesenteric lymph nodes were isolated from male C57BL/6 mice (12–18 weeks), homogenized and CD4⁺ cells obtained by magnetic separation. CD4⁺ T-cells were cultured with L. reuteri CM for 4 days. Supernatants from these cultures (secreted factors) were collected after 4 days and used to treat osteoblast cells for 6 hours. (A) cell viability was measured by intracellular ATP levels; (B) BCL2/BAX gene expression ratio; (C) osterix gene expression. Ctrl = control, LR-Ctrl = L. reuteri CM with no cells, T-cell sup = T cells with no treatment. Statistical analysis performed one-way ANOVA with Bonferroni’s multiple comparision test. *p < 0.05, ***p < 0.001, ****p < 0.0001. n = 5–6.