Figure 9
Effect of L. reuteri conditioned media fractions on naïve CD4+ T Cell cytokine expression. Spleens were isolated from male C57BL/6 mice (12–18 weeks) and naïve CD4+ T cells obtained by magnetic separation. CD4+ T cells were cultured with either a) whole or <3kD CM or b) SPE fractionated CM and expression of cytokines analyzed by flow cytometry. SV = SpeedVac. Statistical analysis performed by unpaired t-test. *p < 0.05, **p < 0.01 compared to comparable control. n = 4–5.
