Figure 7 | Scientific Reports

Figure 7

From: TNFα-signal and cAMP-mediated signals oppositely regulate melanoma- associated ganglioside GD3 synthase gene in human melanocytes

Figure 7

Inhibition of phosphorylation of IKK by WDL in melanocytes and melanomas. (a) Melanocytes (LP) were treated with TNFα (10 ng/mL), TNFα and WDL (20 μM), or WDL alone for 24 h. Data represent means ± s.d. (n = 3). (b) SK-MEL-28, SK-MEL-23, MeWo, and SK-MEL-130 were treated with TNFα (10 ng/mL), TNFα and WDL (20 μM), or WDL alone for 24 h. Phosphorylation levels of IKKs (p-IKKα/β) were examined by western blotting with anti-phospho-IKKα/β (Ser176/180) antibody. Band intensities of p-IKKα/β were measured and presented after correction by those of IKKα/β. Four kinds of samples from melanocytes and individual melanoma cell lines (TNFα (−)/WDL (−), TNFα (+)/WDL (−), TNFα (+)/WDL (+), TNFα (−)/WDL (+)) were applied on the same gel and immunoblotted. Data represent means ± s.d. (n = 5–7). Statistical analysis was performed by the two-tailed Student’s t-test (*P < 0.05; **P < 0.01). ns = not significant.

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