Figure 4 | Scientific Reports

Figure 4

From: Solution structure, glycan specificity and of phenol oxidase inhibitory activity of Anopheles C-type lectins CTL4 and CTLMA2

Figure 4The alt text for this image may have been generated using AI.

Solution scattering data and model for CTL4/CTLMA2. (a) Sequence alignment for An. gambiae and An. albimanus CTL4 and CTLMA2 with mouse scavenger receptor CTLD (SCRL, PDB ID 2OX9) and porcine surfactant protein D (SP-D, PDB ID 4DN8). Identically conserved residues are highlighted with black. Residues of the Ca2+/glycan binding loop are highlighted in pink. CTL4 basic loop 1 residues are highlighted in blue, CTLMA2 acidic loop 1 residues in red. (b) Molecular model for CTL4 (green) and CTLMA2 (orange). Ca2+/glycan binding loop highlighted in pink. Cysteines in disulfide bonds shown as yellow sticks. Based on proximity of the N-terminal CXC motif to form an intermolecular disulfide, the likely orientation of CRDs in the CTL4/CTLMA2 heterodimer would have outward-facing Ca2+/glycan binding loops and inward-facing charged loops. (c) Small-angle x-ray scattering curve for A. gambiae CTL4/CTLMA2. (inset) Guinier plot (PRIMUS), RG = 24.5 Å. (d) P(r) distribution (GNOM), Dmax = 80 Å. (inset) fit to the scattering curve, RG = 25.4 Å. (e) CTL4/CTLMA2 models arising from a 3-state fit to the scattering curve (MULTIFOXS). One model is aligned with the most probable of 20 ab initio bead models fit to the P(r) distribution (DAMMIF). Measurements derived from a single experiment.

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