Table 1 Calcium binding of CTL4 and CTLMA2 by ITC*.

From: Solution structure, glycan specificity and of phenol oxidase inhibitory activity of Anopheles C-type lectins CTL4 and CTLMA2

 

An. gambiae

An. albimanus

CTL4

CTLMA2

CTL4/CTLMA2

CTL4/CTLMA2

N (sites)

1.00

1 (fixed)

0.36 (0.07)

0.497 (0.003)

KD (µM)

N/A

173 (27)

4.9 (0.5)

2.82 (0.01)

ΔH (kcal/mol)

N/A

−12 (2)

−23 (4)

−12.1 (0.1)

ΔG (kcal/mol)

N/A

−5.1 (0.1)

−7.2 (0.1)

−7.57

−TΔS (kcal/mol)

N/A

7 (2)

16 (4)

4.57

  1. *Values indicate average (standard deviation) from three independent experiments.
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