Table 2 Percentage of embryos containing unedited WT alleles or only indels non-multiple of 3. Different superscript letters indicate significant differences based on Chi-square test (p < 0.05) between the four microinjection groups.
From: Strategies to reduce genetic mosaicism following CRISPR-mediated genome edition in bovine embryos
Group | No. of edited embryos | No. of edited non-mosaic embryos (%) | No. of embryos containing an unedited WT allele (%) | No. of embryos containing only indels non-multiple of 3 (%) |
|---|---|---|---|---|
RNP-injected 0 hpi | 20 | 14 (70.0)a | 8 (40.0)a,b | 3 (15.0) |
mRNA-injected 0 hpi | 20 | 14 (70.0)a | 6 (30.0)a | 6 (30.0) |
mRNA-injected 10 hpi | 20 | 13 (65.0)a | 6 (30)a | 6 (30.0) |
RNP-injected 20 hpi | 5 | 0 (0)n.d. | 4 (80.0)n.d. | 0 (0) |
mRNA-injected 20 hpi | 10 | 0 (0)b | 8 (80.0)b | 1 (10.0) |
