Table 2 Details of selected candidate reference genes.

ACTB

Beta actin

Cytoskeletal structural protein

Commonly accepted reference gene^25,32,86

Myxobolus cerebralis^32,33

AHC1

Adenosylhomocysteinase

Homocystein synthesis protein

Corals²⁵

EF2

Eukaryotic Translation Elongation Factor 2

Nascent protein synthesis protein

Commonly accepted reference gene^67,68

GAPDH

Glyceraldehyde-3-Phosphate Dehydrogenase

Metabolic protein (glycolytic enzyme)

Commonly accepted reference gene²³

HPRT 1

Hypoxanthine-Guanine Phosphoribosyltransferase 1

Purine nucleotide synthesis protein

Commonly used for humans²²

RPB2

DNA-directed RNA polymerase II

RNA polymerase II transcription machinery protein

Humans⁸⁷

18S rRNA

18S ribosomal RNA gene SSU

Ribosome structural protein

Commonly used refrence gene^23,88,

Myxobolus cerebralis³¹

28S rRNA

28S ribosomal RNA gene LSU

Ribosome structural protein

Commonly used reference gene⁸⁹