Figure 4

Simulation results of collective cell behaviors within different microsystems. (a) Cells seeded in opposite inlets and growing through a single-channel fluidic microsystems. Selected representative iterations are shown. (b) Detailed view of another simulation within the same single-channel device, in which two cells types -healthy (green) and invasive (blue)- are seeded and in which the addition of a drug (pink) and its diffusion, interacting just with the invasive cells, is also modeled. Selected iteration when the drug is added and aspect after addition of the drug. (c) Different cells seeded in radially placed inlets and proliferating along the radial channels to meet in a central chamber. Healthy (green) and invasive (blue) cells with different growth rates can be appreciated and the incorporation of a drug (pink) and its diffusion is also modeled. Selected representative iterations are shown. In all cases, the red points represent dead cells according to the defined death probabilities. For each model different representative iterations are selected to illustrate the dynamic process. Each pixel corresponds to a single cell (typically 20 × 20 μm²). Scale bars: 2 mm.