Figure 8
Recording chamber and life support system. (A) Assembled recording chamber with temperature measurements during experimental protocols. (A1) Base plate, MEA, and assembled chamber block. (A2) Side view of chamber with thermocouple placement for medium and chamber temperature measurements. Medium temperatures were measured through a Luer port at position 1 in separate tests. Chamber volume: 5 ml. (A3) Medium temperature before, during, and after system disconnect with correlated mean spike and burst rates. (1) Recording and life support disconnect; (2) impact; (3) life support and amplifier reconnect (4). Amplifier power on. Note that the recording period was started only when the temperature had reached reference. (B) Life support system. A peristaltic pump moved medium at a rate of 0.5 ml/min from a heated reservoir containing 15 ml of medium through the chamber and back into the reservoir. 10% CO2 (10 ml/min) was provided by an Aalborg gas controller from mixing 100% CO2 with filtered room air compressed in an accumulator at 20 psi. A DC temperature control system maintained the chamber temperature at 37 ± 1 °C via four 4-ohm power resistors. Total system medium volume: 20 ml.
