Table 2 Primers used in qPCR and amplicon preparation for microarray and Illumina sequencing analyses.
Primer | Sequence (5′-3′)a | Sense | Target | Ref. |
|---|---|---|---|---|
eub338f | ACTCCTACGGGAGGCAGCAG | forward | 16S rRNA gene, bacteria | |
eub518r | ATTACCGCGGCTGCTGG | reverse | ||
act235f | CGCGGCCTATCAGCTTGTTG | forward | 16S rRNA gene, actinobacteria | |
StrepF | GCAGGACGCTCACCAGGTAGT | forward | txtB gene | |
StrepR | ACTTCGACACCGTTGTCCTCAA | reverse | ||
T7-pA | TAATACGACTCACTATAG-AGAGTTTGATCCTGGCTCAG | forward | 16S rRNA gene, bacteria | |
pH | AAGGAGGTGATCCAGCCGCA | reverse | ||
CS1_515F | ACACTGACGACATGGTTCTACA-GTGCCAGCMGCCGCGGTAA | forward | 16S rRNA gene, bacteria | |
CS2_806R | TACGGTAGCAGAGACTTGGTCT-GGACTACHVGGGTWTCTAAT | reverse | ||
CS1_ARC344F | ACACTGACGACATGGTTCTACA-AC-GGGGYGCAGCAGGCGCGA | forward | 16S rRNA gene, archaea | |
CS2_Arch806R | TACGGTAGCAGAGACTTGGTCT-GG-ACTACVSGGGTATCTAAT | reverse | ||
CS1_Euk1391F | ACACTGACGACATGGTTCTACA -CG -GTACACACCGCCCGTC | forward | 18S rRNA gene, eukaryotes | |
CS2_EukBr | TACGGTAGCAGAGACTTGGTCT-CA-TGATCCTTCTGCAGGTTCACCTAC | Reverse |
