Figure 2
Results from MS experiments. (A) Native ESI mass spectra collected in the presence of 14 µM CIB2 in 200 mM ammonium acetate, pH 6.8. Collision energy was set to 60 V. (B,C) MALDI-TOF-MS measurements of cross-linked CIB2 samples with peptides α7B_M (B) and α7B_Scrb (C). 10 µM CIB2 was cross-linked with 50-fold molar excess of DSBU with increasing concentrations (5 μM, red line; 10 μM, blue line; 20 μM, green line) of α7B_M (B) and α7B_Scrb (C) peptides in the presence of 1 mM Mg2+ and 2 mM Ca2+. Between 1000 to 3000 laser shots were accumulated to one MALDI-TOF mass spectrum. CIB2 without DSBU cross-linker was included as internal control (black line).
