Figure 5
From: Pluronic gel-based burrowing assay for rapid assessment of neuromuscular health in C. elegans
Distribution of wild-type animals during burrowing. The gel height in a well was divided into three vertical layers to monitor the number of animals in each. (a) A front view of a well showing three vertical layers of i, ii, and iii. Animal distribution in different vertical layers of the gel (b) in the presence of food source, N = 33 animals and (c) in the absence of food source, N = 30 animals. h = H represents the top of the gel where the attractant was placed (See Fig. 1a). Assay conditions were 26% w/w PF-127, H = 0.9 cm and 100 mg/mL E. coli.
