Figure 3 | Scientific Reports

Figure 3

From: Mutations in Glucan, Water Dikinase Affect Starch Degradation and Gametophore Development in the Moss Physcomitrella patens

Figure 3

Examination of GWD in P. patens. (a) Protoplasts expressing PpGWDa or PpGWDb fused to GFP. Scale bar is 5 µm. (b) PCR analysis from gDNA demonstrating the presence of mutant alleles in Ppgwda, Ppgwdb or double mutant (DM) lines. Amplicons were separated on a 1% (w/v) agarose gel. λ-PstI represent λ phage DNA digested with PstI. (c) Semi-quantitative RT-PCR analysis of PpGWD1a, PpGWD1b or Actin expression in the wild-type (WT), Ppgwd1a, Ppgwd1b and DM experimental lines. NTC designates the no template control. Original gels are shown in Supplementary Fig. 2. (d) Glucose 6-phosphate amounts in starch from the wild-type and mutant lines. Data represent means of three independent digestions of pooled starch samples ± SEM. Letters represent groups with similar means at the 5% significance level as determined using the Bonferroni-Holm post hoc test following a one-way analysis of variance. Equal variance was determined using Levene’s test.

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